Isolation and enrichment of skin stem cells, especially CD34+cells along with dermal and epidermal cells, using two-stage microfluidic technology can now generate significant levels of hair follicles and sebaceous glands. The microfluidic devices coated with degradable antibody-functionalized hydrogels enables direct isolation and injection of CD34+ skin stem cells without prior labelling, completing the process in about 45 minutes. This technology, when scaled & multiplexed to rapidly separate large amounts of cell mixture, may improve the practicality and efficiency of regenerative therapies and future hair transplantation procedures.